Journal: Experimental neurology

Article Title: DDX3X overexpression decreases dipeptide repeat proteins in a mouse model of C9ORF72-ALS/FTD

doi: 10.1016/j.expneurol.2024.114768

Figure Lengend Snippet: a. Diagram illustrating the AAV9-FLAG-DDX3X and AAV9-GFP constructs used for gene delivery. b. DDX3X delivery in P0-P1 pups by i.c.v injection. Pathological phenotypes were examined in mice at 12 months of age. c. Representative IF images of GFP and DDX3X expression in the cerebral cortex, hippocampus, cerebellum, and spinal cord from mice at 12 months. Scale bar: 25μm.

Article Snippet: C9-BAC mice (FVB/NJ-Tg( C9orf72 )500Lpwr/J) were purchased from Jackson Lab (IMSR_JAX:029099).

Techniques: Expressing, Construct, Injection

Journal: Experimental neurology

Article Title: DDX3X overexpression decreases dipeptide repeat proteins in a mouse model of C9ORF72-ALS/FTD

doi: 10.1016/j.expneurol.2024.114768

Figure Lengend Snippet: a. Representative IHC staining and the quantification of poly-GA, poly-GR and poly-GP in the cerebral cortex of 12-month-old C9-BAC and NT mice after AAV9-GFP/DDX3X expression. Scale bar: 5 μm. Arrows point to DPR aggregations. b. ELISA analysis of poly-GP protein levels in the cortex, spinal cord, and cerebellum. Data are presented as mean ± SEM. At least 4 animals were analyzed by one-way ANOVA test with Turkey’s multiple comparisons. *P<0.05; **P<0.01. n.s, no statistically significant difference.

Article Snippet: C9-BAC mice (FVB/NJ-Tg( C9orf72 )500Lpwr/J) were purchased from Jackson Lab (IMSR_JAX:029099).

Techniques: Over Expression, Immunohistochemistry, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Experimental neurology

Article Title: DDX3X overexpression decreases dipeptide repeat proteins in a mouse model of C9ORF72-ALS/FTD

doi: 10.1016/j.expneurol.2024.114768

Figure Lengend Snippet: a. Representative images of p62 inclusion in cortical neurons. Scale bar, 20μm. b. Quantitative analysis of the proportion of p62 puncta-positive neurons in the cortex. At least 3 animals were included in each group. One-way ANOVA test with Turkey’s multiple comparisons was performed to analyze the differences among groups. Data are presented as mean ± SEM. *P<0.05; **P<0.01.

Article Snippet: C9-BAC mice (FVB/NJ-Tg( C9orf72 )500Lpwr/J) were purchased from Jackson Lab (IMSR_JAX:029099).

Techniques: Over Expression

Journal: Experimental neurology

Article Title: DDX3X overexpression decreases dipeptide repeat proteins in a mouse model of C9ORF72-ALS/FTD

doi: 10.1016/j.expneurol.2024.114768

Figure Lengend Snippet: a. Representative IF images NeuN (left) and quantification of NeuN positive cells (right) in the cortex of C9-BAC and NT mice. Scale bar, 200μm. b. Representative images (left) and quantification of NeuN positive cells (right) in the hippocampus. Scale bar, 200μm. n=3 for each group. One-way ANOVA test with Turkey’s multiple comparisons was performed to analyze the differences among groups. Data are presented as mean ± SEM. n.s, no statistically significant difference.

Article Snippet: C9-BAC mice (FVB/NJ-Tg( C9orf72 )500Lpwr/J) were purchased from Jackson Lab (IMSR_JAX:029099).

Techniques: Over Expression

Journal: Experimental neurology

Article Title: DDX3X overexpression decreases dipeptide repeat proteins in a mouse model of C9ORF72-ALS/FTD

doi: 10.1016/j.expneurol.2024.114768

Figure Lengend Snippet: a. Representative IF images of GFAP and Iba1 in the cortex. b. Quantification of GFAP and Iba1 positive cells in cortex. c. Representative IF images of GFAP and Iba1 in the hippocampus. d. Quantification of GFAP and Iba1 positive cells in the hippocampus. Scale bar, 50μm. n=3–4 for each group. Statistical difference was analyzed by one-way ANOVA test. Data are presented as mean ± SEM. n.s, no statistically significant difference.

Article Snippet: C9-BAC mice (FVB/NJ-Tg( C9orf72 )500Lpwr/J) were purchased from Jackson Lab (IMSR_JAX:029099).

Techniques: Over Expression